Welcome to the forum.
Here are a couple of recnet threads on Samui with relevant info:
http://ladyboyforum....-can-do-couple/
http://ladyboyforum....0616-koh-samui/
And an older thread with some nice Samui info:
http://ladyboyforum....9816-koh-samui/
I spent 2012 living in Samui. It definitely has a bar scene and ladyboys around, but nothing in comparison to Bangkok or Patttaya, or even Phuket.
Samui today is only a shadow of what it was back in 2004 (my first visit), and it's lost a lot of it's charm and island vibe, with Bophut, Lamai and Chaweng now over crowded tourists and backpackers (and Thais cashing in). But it still has some stunning beaches, especially if you are willing to rent a bike or car and explore.
If you are looking for a combi of ladyboys and paradise beaches I'd opt for Phuket over Samui, or if you just want ladyboys with a sea view I'd go for Pattaya.